This data set contains collection records for Scaptodrosophila aclinata for 24 locations where flies were sampled directly from Hibiscus flowers in 1995 (Fig. 1). Hibiscus species, coordinates and altitude are reported for each location. When using this data, please cite the original publication: Barker, J S F (2005). Population structure and host-plant specialization in two Scaptodrosophila flower-breeding species. Heredity 94: 129-138