This data file contains frequencies of the In(2L)t, In(2R)NS, In(3L)Payne, In(3R)Payne and the In(3R)C inversion of 12 Drosophila melanogaster populations collected in 1978-1979 (extracted from Table 1). When using this data, please cite the original publication: Knibb, W. R., Oakeshott, J. G. and Gibson, J. B. (1981). Chromosome inversion polymorphism in Drosophila melanogaster. I. Latitudinal clines and associations between inversions in Australasian populations. Genetics 98: 833-847