This data set contains frequencies of the inversion In(3R)Payne for 46 Drosophila melanogaster locations collected during 1978-1982 and for 34 locations collected during 2002-2004 (Fig. 1). Note longitudes in data are approximate. When using this data, please cite the original publication: Umina, P. A., Weeks, A. R., Kearney, M. R., McKechnie, S. W., and Hoffmann, A. A. (2005). A Rapid Shift in a Classic Clinal Pattern in Drosophila Reflecting Climate Change. Science 308: 691-693