This data file contains heat knockdown time in minutes (corrected for different runs) for individual females of 8 Drosophila melanogaster populations collected in 2001 and kept under three seasonal conditions (Fig. 1). Note longitudes in data are approximate. When using this data, please cite the original publication: Hoffmann, A. A., Shirriffs, J. and Scott, M. (2005). Relative importance of plastic vs genetic factors in adaptive differentiation: geographical variation for stress resistance in Drosophila melanogaster from eastern Australia. Functional Ecology 19: 222-227