The dataset includes a Matlab (“.mat”) file for each experimental trace measured in the optical tweezers. Each file contains a single structure with the name “Data” that has the following fields: Data.time – An array containing the time (in seconds, at 2.5 kHz) Data.contour – An array containing the measured contour length (in bps, at 2.5 kHz) Data.force – An array containing the measured force (in pN, at 2.5 kHz) Data.Protein – A string with the name of the protein used Data.DNAconstruct – A string with the type of construct (Hairpin, symmetric, RecB, and RecD) Data.ATP – The ATP concentration (uM) Data.ProteinConcentration – The protein concentration(nM)