Figure 5 - Figure supplement 1 - Peptides eluted from HeLa-S+IFNy and HeLa-S-shTAPBPR+IFNy

Peptide-MHC class I complexes were isolated by affinity chromatography using W6/32 from IFN-γ treated HeLa-S cells and IFN-γ treated HeLa-S cells depleted of TAPBPR using shRNA. Eluted peptides were analysed using LC-MS/MS. Peptides were assigned as HLA-A*68:02 and HLA-B*15:03 binders based on their peptide motifs using the online programme NetMHC.