IMR90 overexpressing MyoD in differentiation medium

Human fibroblasts IMR90 transfected with doxycycline inducible mouse MYOD were cultured in growth media (GM, DMEM high glucose, 10% FBS). MYOD was induced with doxycycline treatment 24 hrs in GM and 72-96 hrs in differentiation media (DM, DMEM high glucose, 2% HS, ITS) prior collection.