Pollen tube growth_Briscoe Runquist et al

We performed intra- and intersubspecific crosses in a greenhouse for 2 sympatric populations of Clarkia xantiana. We applied single-donor pollen and noted the time that we pollinated and the time at which we fixed the style (4-6 hours). We then measured the length of the longest pollen tube and the length of the style and used the data to obtain growth rates and proportional distance.