Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field] See ReadMe file for culture and staining information.