Video 9. Three-dimensional block-face SEM images of Mm infected tail fin.

A double transgenic Tg(mpeg1:EGFP) and Tg(lyz:DsRed2) larva infected with M. marinum was fixed and block face SEM imaging was performed on an 80 by 80 µm region of interest using a Quanta FEG 250 with a Gatan 3View Ultramicrotome. The video shows 154 images acquired every 150 nm step size in z-direction (103 nm pixel size) at 10 frames per second. Scale Bar: 10 µm.