This data file contains Frost 6P and 10P allele frequencies (%) of 17 Drosophila melanogaster populations collected in 2005 and genotyped by acrylamide gel electrophoresis (Fig. 3A). Note longitudes in data are approximate. When using this data, please cite the original publication: Hoffmann, A. A., Blacket, M. J., McKechnie, S. W., Rako, L., Schiffer, M., Rane, R. V., Good, R. T., Robin, C., and Lee, S. F. (2012). A proline repeat polymorphism of the Frost gene of Drosophila melanogaster showing clinal variation but not associated with cold resistance. Insect Molecular Biology 21: 437-445