D13C and D15N - Kattegat
datasetposted on 24.11.2019, 11:40 by Hilmar Hinz
White muscle tissue samples from 5-6 individuals of each respective study species were collected for each station. For the flatfish, a 2 x 2 cm piece of white muscle tissue was taken with the skin removed approximately 2 cm to the right of the pectoral fin and 1 cm above the lateral line. For the Nephrops, white muscle tissue was extracted from the first tail segment with shell, digestive and nerve tract removed. Due to logistical constraints of the sampling, all samples from t were preserved in 100% pure ethanol for transport to the laboratory. In the laboratory all tissue samples were oven dried for 24 hours at 75°C. Each individual tissue sample was ground to a homogenous powder using an agate pestle and mortar. Subsequently, 1.5-2.0 mg of the resulting powder was weighed into tin capsules for stable isotope analysis. Tissue samples were combusted for C and N isotope compositions using continuous flow isotope ratio mass spectrometry (CF-IRMS) THERMO delta X PLUS mass spectrometer (Scientific Technical Services - University of the Balearic Islands). The isotope mass spectrometer was operating in dual mode measuring δ15N and δ13C in the same sample. Additionally C/N ratios are provided.