Data from Mattes 2018
datasetposted on 01.08.2018, 03:30 by Benjamin Mattes, Yonglong Dang, Gediminas Greicius, Lilian Tamara Kaufmann, Benedikt Prunsche, Jakob Rosenbauer, Johannes Stegmaier, Ralf Mikut, Suat Özbek, Gerd Ulrich Nienhaus, Alexander Schug, David M. Virshup, Steffen Scholpp
Kinase Screen and automated image-analysis identifies the receptor tyrosine kinase Ror2 as a potential cytoneme regulator upstream of Cdc42. Wnt8a-GFP and a membrane bound mCherry was co-transfected with kinase library genes in Pac2 cells in a 96-well plate. Images were acquired automatically and analyzed for filopodia length and numbers by a filopodia detection software. Automated Image analysis software detects and counts filopodia of single cells using the memCherry signal and quantifies their length by automatically tracing the tips back to the cell body. Table shows transfected kinase genes and their relative filopodia number/cell and length.