Figure 3 - Source Data 2
datasetposted on 21.11.2019, 10:45 by Asher Preska Steinberg, Sujit S. Datta, Thomas Naragon, Justin C. Rolando, Said R. Bogatyrev, Rustem F. Ismagilov
This zip archive contains the original z-stacks used for Figure 3, panels E-F. These are provided as TIF files. All imaging parameters are included in the image metadata. They are labeled as follows (if multiple technical replicates were done it is indicated): A7PS263_s6_t1 = 30 µm-filtered, technical replicate #1; A7PS263_s7_t1 = 0.45 µm-filtered; A7PS263_s8_t1 = 100 kDa-filtered; A7PS263_s9_t1 = 30 kDa-filtered; A7PS263_s10_t1 = 3 kDa-filtered; A7PS284_s13_t1 = HBSS; A7PS285_s3_t1 = 30 µm-filtered, technical replicate #2; A7PS285_s4_t1 = 0.45 µm-filtered, technical replicate #2; A7PS285_s7_t1 = 30 µm-filtered, technical replicate #3; A7PS285_s8_t1 = 0.45 µm-filtered, technical replicate #3. Additionally, this file contains .csv files of all the statistics on particle sizes collected by the ImageJ macro. These are labeled in the format “zstackname_automated_counting_statistics”, where “zstackname” is the title of the associated z-stack. It also contains a .csv file of the particle # IDs of the singlet particles that were used to get the average singlet size, which was then used to normalize the ECDFs (as explained in detail in Materials and Methods). These are labeled in the format “zstackname_singlet_IDs”, where “zstackname” is the title of the associated z-stack. These .csv files were used in combination with the Jupyter notebooks (also provided with this submission) to generate Figure 3, panels E-F. If multiple technical replicates were performed on a sample, the resulting ECDFs were combined to create one master ECDF for that sample.