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Figure 4 - source data 4

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posted on 21.11.2019, 13:12 by Asher Preska Steinberg, Sujit S. Datta, Thomas Naragon, Justin C. Rolando, Said R. Bogatyrev, Rustem F. Ismagilov
This zip archive contains the original z-stacks used for Figure 4, panel D. These are provided as TIF files. All imaging parameters are included in the image metadata. They are labeled as follows: A7PS290_s17_t1 = 212 mg/mL PEG 3.35 kDa A7PS290_s18_t1 = 106 mg/mL PEG 3.35 kDa A7PS290_s19_t1 = 53.0 mg/mL PEG 3.35 kDa A7PS290_s20_t1 = 26.5 mg/mL PEG 3.35 kDa A7PS290_s21_t1 = 13.2 mg/mL PEG 3.35 kDa A7PS290_s22_t1 = 6.62 mg/mL PEG 3.35 kDa A7PS290_s23_t1 = 3.31 mg/mL PEG 3.35 kDa A7PS290_s24_t1 = 1.66 mg/mL PEG 3.35 kDa A7PS321_s1_t1 = 0.815 mg/mL PEG 3.35 kDa A7PS321_s2_t1 = 0.407 mg/mL PEG 3.35 kDa A7PS321_s3_t1 = 0.204 mg/mL PEG 3.35 kDa A7PS321_s4_t1 = 0.102 mg/mL PEG 3.35 kDa A7PS321_s5_t1 = 0.0509 mg/mL PEG 3.35 kDa Additionally, this file contains .csv files of all the statistics on particle sizes collected by the ImageJ macro. These are labeled in the format “zstackname_automated_counting_statistics”, where “zstackname” is the title of the associated z-stack. It also contains a .csv file of the particle # IDs of the singlet particles that were used to get the average singlet size, which was then used to normalize the ECDFs (as explained in detail in Materials and Methods). These are labeled in the format “zstackname_singlet_IDs”, where “zstackname” is the title of the associated z-stack. These .csv files were used in combination with the Jupyter notebooks (provided with this submission) to generate Figure 4, panel D.






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