Illumina MiSeq data ITS2 Vascular plants
datasetposted on 30.03.2018 by Tobias Guldberg Frøslev, Rasmus Kjøller, Hans Henrik Bruun, Rasmus Ejrnæs, Ane Kirstine Brunbjerg, Carlotta Pietroni, Anders Johannes Hansen, Tobias Frøslev
Datasets usually provide raw data for analysis. This raw data often comes in spreadsheet form, but can be any collection of data, on which analysis can be performed.
Contains 12 fastq files corresponding to R1 and R2 data from 6 Illumina MiSeq 250 bp PE sequencing libraries. 130 soil samples from Denmark were amplified with tagged primers (S2F and ITS4) and build into TruSeq PCR free libraries. Each sample was amplified in triplicates. Fastq files R1A and R1B contain reads from the first replicates, R2A and R2B from the second, etc. Data were produced as part of the Biowide (Biodiversity in width and depth), carryied out in Denmark from 2014-2018) supported by the Villum foundation. The vascular plant data included here was used in the validation of the LULU algorithm for post-clustering curation of OTU tables for reliable biodiversity metrics, as described in the publication. Tagging scheme and other information needed to demultiplex and analyse the data are accessible on the GitHub repository accompanying the publication/r-package.