Suppl dataset 1 RNAseq
datasetposted on 12.11.2019, 09:19 by Renu Srivastava, Zhaoxia Li, Giulia Russo, Jie Tang, Ran Bi, Usha Muppirala, Sivanandan Chudalayandi, Andrew Severin, Mingze He, Samuel Vaitkevicius, Carolyn Lawrence-Dill, Peng Liu, Ann Stapleton, Diane Bassham, Federica Brandizzi, Stephen Howell
The ER stress transcriptome was profiled in maize B73 seedlings treated with 5 g ml-1 TM for 0, 3, 6, 12, 24, 36, and 48 h. (A 48-h mock-treated sample was included.) RNA was extracted from root samples and cDNA libraries were generated and subjected to DNA sequencing. Read sequences from three biological replicates (each with two technical replicates) were aligned to the maize genome v4. Read counts were normalized and differential gene expression was assessed using generalized linear models. Tab 1: Cluster analysis. Tab 2: RPKM values during time course following TM treatment. Tab 3: Mean and standard error of RPKM values.
dc.coverage.spatialTemperate and subtropical zones
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Srivastava R, Li Z, Russo G, Tang J, Bi R, Muppirala U, Chudalayandi S, Severin A, He M, Vaitkevicius SI, Lawrence-Dill CJ, Liu P, Stapleton AE, Bassham DC, Brandizzi F, Howell SH (2018) Response to persistent er stress in plants: a multiphasic process that transitions cells from prosurvival activities to cell death. The Plant Cell 30(6): 1220-1242.