Validation of selected genes obtained from RNA-seq by q-PCR.
datasetposted on 24.11.2019 by Jianrong Li, Kiyomi Abe, Anna Milanesi, Yan-Yun Liu, Gregory A Brent
Datasets usually provide raw data for analysis. This raw data often comes in spreadsheet form, but can be any collection of data, on which analysis can be performed.
Figure S1. Viladation of selected genes obtained from RNA-seq by q-PCR. Primary cortical neurons were exposed to hyoixa (0.2% oxygen) for 7 hours either in the presence or absence of 5nm T3. Total RNA was isolated from cells, reverse transcribed, and evaluated by q-PCR. The q-PCR was performed in triplicates and three housekeeping genes were included as controls (GAPDH, B-actin and cyclophilin A).