Validation of selected genes obtained from RNA-seq by q-PCR.
datasetposted on 24.11.2019, 21:06 by Jianrong Li, Kiyomi Abe, Anna Milanesi, Yan-Yun Liu, Gregory A Brent
Figure S1. Viladation of selected genes obtained from RNA-seq by q-PCR. Primary cortical neurons were exposed to hyoixa (0.2% oxygen) for 7 hours either in the presence or absence of 5nm T3. Total RNA was isolated from cells, reverse transcribed, and evaluated by q-PCR. The q-PCR was performed in triplicates and three housekeeping genes were included as controls (GAPDH, B-actin and cyclophilin A).