V781-PG9 engineered Ramos cells after HIV C108 SOSIP enrichment followed by two rounds of selection using HIV WITO SOSIP. Immunoglobulin heavy chain variable sequences from RT-PCR and amplification of cell mRNA performed using gene specific primers. TruSeq LT amplicon sequenced using 300x300 paired end reads on the Illumina MiSeq. This is read 2 (R2)