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Demultiplexed_and_Trimmed_AllSamples.zip (13.75 GB)

demultiplexed & trimmed reads for all samples (n=81)

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posted on 28.03.2018, 16:10 by Lily Lewis, Elisabeth M Biersma, Sarah B Carey, Kent Holsinger, Stuart F McDaniel, Ricardo Rozzi, Bernard Goffinet
Compressed package with demultiplexed and trimmed RADseq data for 81 samples (i.e. 81 .fastq.gz files representing all samples listed in Appendix S1). Demultiplexing and trimming was done in PyRAD v. 2.17 (Eaton 2014). Reads were de-multiplexed allowing for a maximum of two mismatches in each eight bp barcode, and restriction enzyme cut sites and adapter sequences were removed. .fastq.gz files are named as such: Sample ID-Collection#.fastq.gz. For example, Nepal_1_1101.fastq.gz is for the sample identified as "Nepal_1" in the associated publication, which was also assigned collection number 1101. The sample ID provides information on the general collection locality.

History

dc.format.extent

14763491748

dc.identifier.uri

http://hdl.handle.net/10255/dryad.143820

dc.coverage.spatial

Global, Northwestern North America, South America, Magellanic sub-Antarctic ecoregion

dc.coverage.temporal

Miocene - Pleistocene

dwc.ScientificName

Splachnaceae, Bryopsida

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