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Live widefield JF635-HaloTag-NLS stage 5 embryo

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posted on 19.11.2019 by Albert Tsai, Anand K. Muthusamy, Mariana R. P. Alves, Luke D. Lavis, Robert H. Singer, David L. Stern, Justin Crocker
Wide field imaging in the anterior region of a live stage 5 Drosophila melanogaster embryo expressing HaloTag-NLS. The HaloTag-NLS protein is unstable and degraded after translation. The embryo is injected with JF635 linked to a HaloTag ligand 2 hours after egg-laying. The embryo is epi-illuminated with a 633 nm laser and videos are captured with an exposure time of 50 ms. Video is played at 10 frames per second.

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dc.format.extent

393232116

dc.identifier.uri

http://hdl.handle.net/10255/dryad.162061

dwc.ScientificName

Drosophila melanogaster

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