HaloTag-NLS.avi (375.02 MB)

Live widefield JF635-HaloTag-NLS stage 5 embryo

Download (375.02 MB)
media
posted on 19.11.2019, 12:35 by Albert Tsai, Anand K. Muthusamy, Mariana R. P. Alves, Luke D. Lavis, Robert H. Singer, David L. Stern, Justin Crocker
Wide field imaging in the anterior region of a live stage 5 Drosophila melanogaster embryo expressing HaloTag-NLS. The HaloTag-NLS protein is unstable and degraded after translation. The embryo is injected with JF635 linked to a HaloTag ligand 2 hours after egg-laying. The embryo is epi-illuminated with a 633 nm laser and videos are captured with an exposure time of 50 ms. Video is played at 10 frames per second.

History

dc.format.extent

393232116

dc.identifier.uri

http://hdl.handle.net/10255/dryad.162061

dwc.ScientificName

Drosophila melanogaster

Licence

Exports