HaloTag-binding%20deficient%20Ubx.avi (750.03 MB)

Live widefield JF635-HaloTag-Ubx(R3A N51A) stage 5 embryo

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posted on 19.11.2019 by Albert Tsai, Anand K. Muthusamy, Mariana R. P. Alves, Luke D. Lavis, Robert H. Singer, David L. Stern, Justin Crocker
Wide field imaging in the anterior region of a live stage 5 Drosophila melanogaster embryo expressing HaloTag-Ubx(R3A N51A). This Ubx is DNA-binding deficient. The embryo is injected with JF635 linked to a HaloTag ligand 2 hours after egg-laying. The embryo is epi-illuminated with a 633 nm laser and videos are captured with an exposure time of 50 ms. Video is played at 10 frames per second.

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dc.format.extent

786460116

dc.identifier.uri

http://hdl.handle.net/10255/dryad.162063

dwc.ScientificName

Drosophila melanogaster

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