Live widefield JF635-HaloTag-Ubx(R3A N51A) stage 5 embryo
mediaposted on 19.11.2019, 12:35 by Albert Tsai, Anand K. Muthusamy, Mariana R. P. Alves, Luke D. Lavis, Robert H. Singer, David L. Stern, Justin Crocker
Wide field imaging in the anterior region of a live stage 5 Drosophila melanogaster embryo expressing HaloTag-Ubx(R3A N51A). This Ubx is DNA-binding deficient. The embryo is injected with JF635 linked to a HaloTag ligand 2 hours after egg-laying. The embryo is epi-illuminated with a 633 nm laser and videos are captured with an exposure time of 50 ms. Video is played at 10 frames per second.