Live widefield JF635-HaloTag-Ubx(R3A N51A) stage 5 embryo
mediaposted on 19.11.2019 by Albert Tsai, Anand K. Muthusamy, Mariana R. P. Alves, Luke D. Lavis, Robert H. Singer, David L. Stern, Justin Crocker
Media is any form of research output that is recorded and played. This is most commonly video, but can be audio or 3D representations.
Wide field imaging in the anterior region of a live stage 5 Drosophila melanogaster embryo expressing HaloTag-Ubx(R3A N51A). This Ubx is DNA-binding deficient. The embryo is injected with JF635 linked to a HaloTag ligand 2 hours after egg-laying. The embryo is epi-illuminated with a 633 nm laser and videos are captured with an exposure time of 50 ms. Video is played at 10 frames per second.