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Script for extracting DNA sequences flanked by MiFish primer-match regions.
online resourceposted on 12.11.2019, 16:20 by Masaki Miya, Yukuto Sato, Tsukasa Fukunaga, Tetsuya Sado, Jan Poulsen, Keiichi Sato, Toshifumi Minamoto, Satoshi Yamamoto, Hiroki Yamanaka, Hitoshi Araki, Michio Kondoh, Wataru Iwasaki
For each of the primer pair, the region with the smallest hamming distance is found in each DNA sequence. If the smallest hamming distance is larger than 3, such sequences are ignored because existence of indels or many mutations is assumed in primer-match regions. For those ignored sequences, manual extraction needs to be conducted afterwards.